Voice Cast
- Manny - Ray Romano
- Sid - John Leguizamo
- Diego - Denis Leary
- Crash - Seann William Scott
- Eddie - Josh Peck
- And Queen Latifah as Ellie
- Lone Gunslinger Vulture - Will Arnett
- Fast Tony - Jay Leno
- Scrat - Chris Wedge
- Dung Beetle Dad - Peter Ackerman
- Glypto Boy (Billy)/Beaver Girl - Caitlin Rose Anderson
- Rhino Boy/Beaver Boy - Connor Anderson
- Mr. Start - Joseph Bologna
- Elk Boy - Jack Crocicchia
- Condor Chick - Peter De Sève
- Start Girl - Nicole DeFelice
- Diatryma Mom - Debi Derryberry
- Start Dad - Marshall Efron
- Stu/Male Ox - Tom Fahn
- Molehog Grandpa/Shovelmouth Male - Jason Fricchione
- Traffic Vulture - James Edmund Godwin
- Beaver Dad - George Jacobs
- Female Mini Sloth/Dung Beetle Mom - Clea Lewis
- Glyptodon - Brian McFadden
- Shovelmouth Boy - Jansen Panettiere
- Start Boy 1 - Gregory Romano
- Start Boy 2 - Matthew Romano
- Aardvark Dad - Stephen Root
- Dodo - Carlos Saldanha
- Molehog Kid 1 - Manoela Scarpa Saldanha
- Molehog Kid 2 - Sofia Scarpa Saldanha
- Diego's Bird Girl - Reyna Shaskan
- Freaky Male/Elk Dad - James Sie
- Aardvark Mom - Cindy Slattery
- Female Ox - Mindy Sterling
- James - Alex Sullivan
- Mrs. Start - Renee Taylor
- Cholly - Alan Tudyk
Additional Voices
- Emily Anderson
- Jack Angel
- Shaun Baumel
- Bob Bergen
- Claudia Besso
- Kyle J. Bogert
- Rodger Bumpass
- Paul Butcher
- Blanca Camacho
- John Cygan
- Jennifer Darling
- Olivia DeLaurentis
- Harrison Fahn
- Bill Farmer
- Crispin Freeman
- Spencer Lacey Ganus
- Jess Harnell
- Tim Kirkpatrick
- David H. Kramer
- Sherry Lynn
- Danny Mann
- Laura Marano
- Madeleine Martin
- Matthew P. McCarthy
- Mickie McGowan
- Laraine Newman
- Jan Rabson
- Zack Shada
- Thomas Sharkey
- Ross Simanteris
- Rachel Stand
- Ariel Winter
